Conversion of Indole-3-Ethanol to Indole-3-Acetic Acid in Cucumber Seedling Shoots

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Conversion of indole-3-ethanol to indole-3-acetic Acid in cucumber seedling shoots.

Indoleethanol-(14)C was applied to intact cucumber seedlings and to hypocotyl segments. The presence of indoleacetic acid-(14)C in tissue extracts was demonstrated by thin layer radiochromatography. There was no evidence of conversion of indoleacetic acid to indoleethanol. It is suggested that the growth-promoting activity of indoleethanol is due to its conversion to indoleacetic acid.

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Conversion of endogenous indole-3-butyric acid to indole-3-acetic acid drives cell expansion in Arabidopsis seedlings.

Genetic evidence in Arabidopsis (Arabidopsis thaliana) suggests that the auxin precursor indole-3-butyric acid (IBA) is converted into active indole-3-acetic acid (IAA) by peroxisomal beta-oxidation; however, direct evidence that Arabidopsis converts IBA to IAA is lacking, and the role of IBA-derived IAA is not well understood. In this work, we directly demonstrated that Arabidopsis seedlings c...

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Indole-3-Acetic Acid and Flowering.

Science, a weekly journal, Is published each Friday by the American Association for the Advancement of Science at The Business Press, Incorporated, N. Queen St. and McGovern Ave., Lancaster, P'a. Founded In 1880. it has been since 1900 the official publication of the AAAS. Editorial and Advertising Offices, 1515 Massachusetts Avenue, N.W.. Washington 5, D. C. Telephone, EXecutive 80 or 661. Cab...

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The Nitrilase ZmNIT2 converts indole-3-acetonitrile to indole-3-acetic acid.

We isolated two nitrilase genes, ZmNIT1 and ZmNIT2, from maize (Zea mays) that share 75% sequence identity on the amino acid level. Despite the relatively high homology to Arabidopsis NIT4, ZmNIT2 shows no activity toward beta-cyano-alanine, the substrate of Arabidopsis NIT4, but instead hydrolyzes indole-3-acetonitrile (IAN) to indole-3-acetic acid (IAA). ZmNIT2 converts IAN to IAA at least se...

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Isolation of Indole-3-ethanol Oxidase from Cucumber Seedlings.

Previous work in this laboratory has shown that cucumber (Cucumis sativus L.) seedlings contain large amounts, relative to other indolic compounds, of extractable indole-3-ethanol (IEt); tracer studies have established that IEt is metabolized to IAA. We have now succeeded in isolating an enzyme from these seedlings which catalyzes the oxidation of IEt to indole-3-acetaldehyde (IAAld). The ident...

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ژورنال

عنوان ژورنال: Plant Physiology

سال: 1967

ISSN: 0032-0889,1532-2548

DOI: 10.1104/pp.42.8.1091